Protein running, transfer and blotting buffer
For Western Blotting
Running Buffer 10X.
35 mM SDS (10.08 g)
250 mM Tris (30.3 g)
1,92 M glycine (144g)
In one liter volume. Dilute to 1X before use.
25 mM Tris (3.03 g)
192 mM glycine (14.4g)
In 800 mL final volume of water. Add 200 mL of methanol 100%. Keep at 4C.
Antibody wash buffer (make fresh)
20 mM Tris pH 7,5 (20 mL of a 1L solution)
150 mM NaCl (30 mL of a 5M solution)
0.02 % Tween 20
Antibody incubation/hybridization buffer (make fresh)
TBS-T + 2.5% defatted dry milk.