Soil selection of transgenics using phosphinothricin/glufosinate/Basta

  1. Weigh between 0.1 and 0.2 gram of seeds and vernalize in water for 48h.
  2. Resuspend seeds in 200ml water, using a 10 ml pipette dispense seeds on a flat.
  3. Place flat in growth room with a clear lid for a week.
  4. Remove lid.
  5. Spray 3-time weekly with 15 mg/L of herbicide.
  6. Resistant plant will appear after 2 weeks.
  7. These can either be transfer to a new flat or spraying can continue until all non-reistant plant die.

TO VIEW WHAT THE PLANTS SHOULD LOOK LIKE AT THE DIFFERENT STEPS YOU CAN VISIT THIS PAGE: http://www.germainhugo.com/lab-members/caroline-mireault/