FTA paper protocol.

To amplify genomic DNA from Arabidopsis.

  1. Cut a small piece of leaf.
  2. Place the leaf on FTA paper, cover with parafilm, press hard with a glass test tube to make the leaf print.
  3. Dry sample at RT at least one hour.
  4. Add 50 microliter of FTA reagent to wells of 96 well plates
  5. Take one punch from each leaves and place in FTA reagent for 5 minutes.
  6. Remove liquid.
  7. Rinse disc twice with 200 microliter TE-1 (10 mM Tris pH 8, 0,1 mM EDTA).
  8. Remove liquid
  9. Add master mix and run PCR.

 

FTA solution (can be made as a 10X stock. Add Tween after autoclaving).

 

10 mM Tris pH 7.5

2 mM EDTA

Tween 20 0.1%