Arabidopsis thaliana quick seeds sterilization

Carry procedure (step 2-6) in a sterile hood.

 

  1. Place 1-250 seeds in 1 ml sterilization solution (1-5% bleach + 0.1% Tween 20) in a 1,5 mL Eppendorf tube. Vortex vigorously until seeds are no longer clumping.
  2. Spin 10 sec @ max speed, remove sup.
  3. Optional (use this step when growing seeds on plates) add 1 mL EtOH 95% vortex vigorously and Spin 10 sec @ max speed, remove sup.
  4. Wash seeds twice with 1 mL sterile ddH2O.
  5. Add 1 mL sterile 0.1% agar.
  6. Place @ 4ºC for 48h.
  7. Resuspend seeds in water and use a 1 mL pipetman to place on soil.